Effects Of Antisense Oligonucleotide To Inos On Hemodynamic

Effects of antisense oligonucleotide to iNOS on hemodynamic and 18 Dijkstra G Moshage H van Dullemen HM Jager-Krikken A Tie- vascular changes induced by LPS Am J Physiol 1998 275 H1078 bosch ATMG Kleibeuker JH Jansen PLM van Goor H Expression 83

Protective Effect of Neurotropin Against Lipopolysaccharide-Induced Hypotension and Lethality Linked to Suppression of Inducible Nitric Oxide Synthase Induction 145-149 1991 11 Hoque AM Papapetropoulos A Venema RC Catravas JD and Fuchs LC Effects of antisense oligonucleotide to iNOS on hemodynamic and vascular changes induced by LPS

Results of in vivo application of AS-ODN targeting iNOS further support the role of NO generated by this high-output enzyme in renal injury 17 x 17 Noiri E Peresleni T Miller F and Goligorsky M S Antisense oligonucleotides to the inducible NOS prevent tubular cell death in ischemic acute renal failure J Clin Invest

Rapid intravenous infusion of GEM 91 a 25-mer phosphorothioate oligonucleotide complementary to the gag site of HIV in the monkey produces transient decreases in peripheral total WBC and neutrophil counts hemoconcentration and a brief increase followed by a prolonged decrease in arterial blood pressure These changes are preceded by and are likely mediated by activation of C5 complement

Sep 01 2012· Much of the prior work that examined the effects of O-GlcNAcylation in the vasculature has focused on the detrimental effects of increased cellular O-GlcNAc levels on vascular function with a particular emphasis on vascular complications of disease …

Antisense oligonucleotides The 18-base phosphorothioated the involvement of iNOS in the relaxant effect of VIP in the isolated gastric smooth muscle cells might be related to the PAPAPETROPOULOS A VENEMA R C CATRAVAS J D FUCHS L C Effects of antisense oligonucleotide to iNOS on hemodynamic and vascular changes induced by LPS

Lipopolysaccharide LPS causes impaired vascular contractility proposed to be mediated by induction of nitric oxide synthase iNOS Antisense AS oligonucleotide inhibits the translation of target mRNA into functional proteins

Preclinical studies looking at the effect of this antisense oligonucleotide on tumor cells have been encouraging In cell culture ISIS 5132 specifically reduced the expression of c-raf kinase mRNA and protein and inhibited cell proliferation in A549 human lung cancer cells The IC 50 for these effects was approximately 100 n m 12 13

Abstract In the fields of neuroendocrinology and behavioral pharmacology antisense technology has been successfully applied in numerous in vivo studies for review see Wahlestedt 1994 Landgraf 1996 The down-regulation of the synthesis of proteins such as enzymes neurotransmitters and neuropeptides and their receptors due to central administration of oligodeoxynucleotides ODNs can

Because of adverse side effects however the use of such antibodies in patients with IBD may be limited Aims An alternative approach to blocking CD154 CD40 interactions by employing a CD40 antisense oligonucleotide ODN was explored

To target iNOS after contusive SCI we previously employed selective pharmacological inhibitors such as 1400W or inos-specific antisense oligonucleotides the latter of which was more effective in acutely reducing iNOS expression and activity limiting neutrophil infiltration and myeloperoxidase

Effect of Inducible Nitric Oxide Synthase on Cerebral Blood Flow after Experimental Traumatic Brain Injury in Mice Article in Journal of Neurotrauma 25 4 299-310 · May 2008 with 11 Reads

Although FXI inhibition does appear to be safe with respect to bleeding the prolonged tissue half-life of antisense drugs makes it necessary to have strategies available that can reverse the effect of the ASO during a bleeding episode or when surgery or other interventional cardiovascular procedures are required on an emergency basis

Effects of antisense oligonucleotide to iNOS on hemodynamic and vascular changes induced by LPS Am J Physiol 1998 275 3 pt 2 H1078 H1083 Google Scholar 24 Misko TP Moore WM Kasten TP Nickols GA Corbett JA Tilton RG McDaniel ML Williamson JR Currie MG Selective inhibition of the inducible nitric oxide synthase by aminoguanidine

Evaluation of the Renal Effects of an Antisense Phosphorothioate Oligodeoxynucleotide in Monkeys Show all authors Complement activation and hemodynamic changes following intravenous administration of phosphorothioate oligonucleotides in the monkey Antisense oligonucleotide inhibitors for the treatment of cancer 1

Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression resulting in adverse side-effects In theory antisense techniques can be very selective and therefore have been investigated as a potential therapeutic approach for IBD L C FuchsEffects of antisense

1 Introduction There has been a recent revival of interest in the use of antisense oligonucleotides to treat neurodegenerative disorders Antisense oligonucleotides are synthetic single stranded strings of nucleic acids between 8 and 50 nucleotides in length that bind to …

Targeting of iNOS with antisense DNA plasmid reduces cytokine-induced inhibition of osteoblastic activity Article PDF Available in AJP Endocrinology and Metabolism 285 3 E614-21 · October 2003

Antisense knockdown of inducible nitric oxide synthase inhibits the relaxant effect of VIP in isolated smooth muscle cells of the mouse gastric fundus

Vascular effects of LPS in mice deficient in expression of the gene for inducible nitric oxide synthase Am J Physiol 1998 275 H416 H421 Crossref Medline Google Scholar 10 Hoque AM Papapetropoulos A Venema RC Catravas JD Fuchs LC Effects of antisense oligonucleotide to iNOS on hemodynamic and vascular changes induced by LPS

The present study investigated the use of antisense oligodeoxynucleotides AS-ODNs to selectively inhibit the expression of iNOS AS-ODNs 1-10 μM inhibited in a time-dependent and dose-dependent manner iNOS activity in RAW 264 7 murine macrophages Maximal inhibitory effect was 90 and control ODNs had little or no effect on NO production

Aug 01 2015· The early development of synthetic oligonucleotides T he landmark discovery of DNA as the hereditary material by Avery et al in 1944 1 followed by the insightful report on the helical structure of DNA 2 paved the way for our current understanding and use of nucleic acids including the development of oligonucleotide ON therapies In this review we will briefly discuss some of the key

Effects of antisense oligonucleotide to iNOS on hemodynamic and vascular changes induced by LPS Article PDF Available in The American journal of physiology 275 3 Pt 2 H1078-83 · October 1998

Abstract To investigate the influence of NF-κB antisense oligonucleotide on transdifferentiation of fibroblast in the pathological process of bleomycin-induced pulmonary fibrosis in mice 6 h before molding of C57BL 6 model of pulmonary fibrosis in mice NF-κB antisense oligonucleotide was injected from caudal vein

Systemically administered 2′-O-methoxyethyl 2′MOE antisense oligonucleotides ASOs accumulate in the kidney and metabolites are cleared in urine The effects of eleven 2′MOE ASOs on renal function were assessed in 2 435 patients from 32 phase 2 and phase 3 trials

To investigate the influence of NF-κB antisense oligonucleotide on transdifferentiation of fibroblast in the pathological process of bleomycin-induced pulmonary fibrosis in mice 6 h before molding of C57BL 6 model of pulmonary fibrosis in mice NF-κB antisense oligonucleotide was injected from caudal vein

Antisense therapy is a form of treatment for genetic disorders or infections When the genetic sequence of a particular gene is known to cause a particular disease it is possible to synthesize a strand of nucleic acid DNA RNA or a chemical analogue that will bind to the messenger RNA mRNA produced by that gene and inactivate it effectively turning that gene off

Oct 14 2014· These are both single-stranded antisense oligonucleotide drugs most commonly known as AONs that together with siRNA a double-stranded oligonucleotide make up at present the therapeutic antisense oligonucleotide field In this paper more emphasis will be put on AONs due to their longer time in development and history of clinical trials

Specific Inhibition of Nitric Oxide Production in Macrophages by Phosphorothioate Antisense Oligonucleotides Author links open overlay panel Hidetoshi Arima Hidetoshi Arima

Apr 03 2009· To characterize the in vivo toxicity of phosphorothioate antisense oligonucleotides against rel A p65 subunit of NF-κB transcription factor forty-eight 6-week-old CD-1 mice were split into 4 groups 6 group receiving vehicle phosphate-buffered saline or doses of 50 100 and 150 mg kg of rel A antisense oligonucleotides intraperitoneally 3 times weekly for 2 weeks