faouzi laachari lipase activity

Faouzi Laachari1 Hajar Maâtaoui1 According to the results we noticed that the lipase activity performance measured by the modified protocol was much improved scoring a maximum of 14

Dec 23 2015· The surface free energy characteristics of untreated and treated cedar wood determined from the contact angle measurements and calculation using the Young Van Oss equations are presented in Table 2 The results showed that the untreated cedar wood surface was qualitatively and quantitatively hydrophobic with values of θ w = 78 1 ± 0 56° and ΔGiwi = −71 99 mJ m 2 0

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The main objective of this work was to identify novel lipases of industrial interest In this paper Aspergillus flavus lipase AFL was isolated from the traditional tannery of Fez city in Morocco it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate NaDC Bile salts showed no inhibitory effect on the lipolytic activity whereas

The Lipase from Staphylococcus aureus 3 Laachari Faouzi El Bergad Fatimazahra Sadiki Moulay Importance of the Ca2 -binding sites in the N-catalytic domain of a family I 3 lipase for activity and stability Protein Engineering Design and Selection 2008 21 12 737CrossRef

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Purification and Characterization of a Novel Thermostable Lipase from Aspergillus Flavus Faouzi Laachari Abstract This newly isolated lipase can be considered as a good candidature in synthesis reactions such as esterification transesterification and interesterification which are used in industrial and biotechnological applications

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1 Introduction Significant rising concern in the field of enzymology on account of wider applications of enzymes in various chemical processes has been increased since a few decades Pliego et al 2015 Due to versatile applications lipases are the third most abundantly used enzymes after proteases and amylases carbohydrases Ülker et al 2011

Oct 28 2000· The Lifshitz−van der Waals acid−base and total surface free energies of various wood species were calculated from contact angle measurements For spruce Picea abies and meranti Shorea spp the following three methods were compared capillary rise in wood powder columns based on the Washburn equation dynamic contact angle measurements according to the Wilhelmy-plate …

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Aspergillus nidulans WG312 strain secreted lipase activity when cultured in liquid media with olive oil as carbon source Highest lipase productivity was found when the mycelium was grown at 30 °C in a rich medium The new enzyme was purified to homogeneity from the extracellular culture of A nidulans by phenyl-Sepharose chromatography and affinity binding on linolenic acid−agarose

Biochemical Characterization of a New Thermostable Lipase from Bacillus Pumilus Strain Faouzi Laachari Soumya Elabed Adel Sayari Iraqui Mohammed El Hassan Harchali Saad Kor Ibnsouda

A specific activity of 1800 U mg was measured on tributyrin or olive oil emulsion as substrate This newly isolated lipase can be considered as a good candidature for industrial and biotechnological applications Keywords Trichosporon coremiiforme lipase purification thermoactive African Journal of Biotechnology Vol 12 28 pp 4503-4511

Nonstop demand for greatly thermostable and thermoactive active lipase encourages the research for the new enzyme sources In this study a strain of Trichosporon coremiiforme was isolated from the traditional tannery in the city of Fez in Morocco lipase production and their lipolytic activity was studied

The main objective of this work was to identify novel lipases of industrial interest In this paper Aspergillus flavus lipase AFL was isolated from the traditional tannery of Fez city in Morocco it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate NaDC Bile salts showed no inhibitory effect on the lipolytic activity whereas

In this paper Aspergillus flavus lipase AFL was isolated from the traditional tannery of Fez city in Morocco it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate NaDC Bile salts showed no inhibitory effect on the lipolytic activity whereas the calcium salts showed a

Lipase activity determination The lipase activity was measured titrimetrically at pH 8 2 and 45°C with a pH-stat under standard conditions using tributyrin 0 25 ml in 30 ml of 2 5 mM Tris HCl pH 8 2 2 mM CaCl 2 2 mM NaDC or olive oil emulsion 10 ml in Turk J Biochem 2015 40 1 8-14 9 Laachari …

Mes remerciements s adressent également à Monsieur F LAACHARI Docteur pour l aide la compréhension ses conseils pour m avoir encouragé et son aide si précieuse Je remercie également Madame S ELABED professeur au CURI Monsieur O EL FARRICHA et Monsieur M A TAHRI JOUTI professeurs à la FSTF d avoir

Mar 01 2015· The activity of lipase was examined within the pH range of 6 0-10 0 and the effect of pH on lipase stability was determined by incubating the lipase fraction in various buffer solutions ranging from 3 0 to 10 0 for 24 h at room temperature Results The results showed that Bacillus pumilus is a strain that produce non-inducible lipase

The main objective of this work was to identify novel lipases of industrial interest In this paper Aspergillus flavus lipase AFL was isolated from the traditional tannery of Fez city in Morocco it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate NaDC Bile salts showed no inhibitory effect on the lipolytic activity whereas

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Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid water interface Faouzi Laachari1 Fatimazahra El Bergadi1 Moulay Sadiki1 Adel Sayari3 Wifak Bahafid1 Soumya Elabed2 Iraqui Mohammed1 and Saad Kor Ibnsouda1 1Laboratoire de Biotechnologie Microbienne Université Sidi Mohamed Ben

A total of 31 filamentous fungi were isolated from deteriorated paper of ancient books Nine of these isolates were screened positive for the ability to degrade carboxymethylcellulose CMC The ability of the nine isolates to produce filter paper activity FPUase in liquid media as well as the effect of temperature and pH were also studied

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Faouzi Laachari Soumya Elabed Adel Sayari Iraqui Mohammed El Hassan Harchali Saad Kor Ibnsouda 2013 Biochemical characterization of a thermoactive and thermostable lipase from a newly olated Trichosporon coremiiforme strain African Journal of …

A specific activity of 1800 U mg was measured on tributyrin or olive oil emulsion as substrate This newly isolated lipase can be considered as a good candidature for industrial and biotechnological applications Keywords Trichosporon coremiiforme lipase purification thermoactive African Journal of Biotechnology Vol 12 28 pp 4503-4511

Biotech Fish processing wastes for microbial enzyme production a review Faouzi Ben Rebah 0 Nabil Miled 0 0 F Ben Rebah N Miled Laboratoire de Biochimie et de Ge ́nie Enzymatique des Lipases ENIS BPW 1173-3038 Sfax Tunisia Fishery processing industries generate large …